Insulin resistance in prepubertal obese children correlates with sex-dependent early onset metabolomic alterations.

摘要: Insulin resistance (IR) is usually the first metabolic alteration diagnosed in obese children and key risk factor for development of comorbidities. The factors determining whether or not IR develops as a result excess body mass index (BMI) are still completely understood. This study aimed to elucidate mechanisms underpinning predisposition toward hyperinsulinemia-related complications by using metabolomic strategy that allows profound interpretation profiles potentially affected IR. Serum from 60 prepubertal (30 girls/30 boys, 50% non-IR each group, but with similar BMIs) were analyzed liquid chromatography–mass spectrometry, gas spectrometry capillary electrophoresis–mass following an untargeted metabolomics approach. Validation was then performed on group 100 additional same characteristics. When without compared, 47 metabolites out 818 compounds (P<0.05) obtained after data pre-processing found be significantly different. Bile acids exhibit greatest changes (that is, approximately 90% increase IR). majority differing between groups lysophospholipids (15) amino (17), indicating inflammation central carbon metabolism most altered processes impaired insulin signaling. Multivariate analysis (OPLS-DA models) showed subtle differences magnified when females alone. Inflammation metabolism, together contribution gut microbiota, signaling sex-specific fashion despite their status.