Immunocapture reverse transcription-polymerase chain reaction combined with nested PCR greatly increases the detection of Prunus necrotic ring spot virus in the peach.

作者: P.R Helguera , R Taborda , D.M Docampo , D.A Ducasse

DOI: 10.1016/S0166-0934(01)00299-3

关键词: Nested polymerase chain reactionIlarvirusPrunus necrotic ringspot virusBiologyBromoviridaeVirusMolecular biologyPolymerase chain reactionReverse transcription polymerase chain reactionReverse transcriptaseVirology

摘要: … A detection system based on nested PCR after IC-RT-PCR (IC-RT-PCR-Nested PCR) was developed to improve indexing of Prunus necrotic ringspot virus … PCR improved detection by …

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