Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions

摘要: In this study, three wheat cultivars (Triticum aestivum L.) Tosun, Bolal (stress tolerant) and Cakmak sensitive) were analysed for the presence of trehalose. Using gas chromatography-mass spectrometry (GC-MS) analysis, trehalose was unambiguously identified in extracts from seeds seedlings different cultivars. The amount quantified by high performance liquid chromatography (HPLC) connected with refractive index detector. Effects drought salt stress on contents studied at seedling level analysis achieved both shoot root tissues. It found that had accumulated under conditions all Furthermore, metabolizing enzymes; trehalose-6-phosphate synthase (TPS) trehalase enzyme activities measured roots shoots control, conditions. TPS activity sharply increased condition highest reached to four times its control condition. increase showed parallelism accumulation Trehalase cultivar decreased conditions, however there no significant change variety. To best our knowledge, is first report enzymes # 2005 Elsevier Ireland Ltd. All rights reserved.