Rapid isolation of metaphase chromosomes containing high molecular weight DNA.

摘要: Metaphase chromosomes with high molecular weight DNA were isolated from Chinese hamster ovary (CHO) cells in a neutral buffer containing polyamines and chelators. The individual, unfixed retained their centromeric secondary constrictions, distinct sister chromatids, complex banding patterns. these was 100-fold larger (2 x 10(8) daltons) than by other procedures. These characteristics indicate preservation during isolation of considerable native structure. In contrast to produced methods, stable storage did not aggregate, thus providing useful material for studies the structure biochemistry individual chromosomes.