Analysis of endotoxin fever in rabbits by using a monoclonal antibody to tumor necrosis factor (cachectin)

摘要: A murine monoclonal antibody to rabbit tumor necrosis factor (TNF; cachectin) was injected intravenously into an endotoxin-treated examine the role of endogenous TNF in fever. Both early and late peaks biphasic fever evoked by endotoxin injection were suppressed antibody. activity detected dose-dependent manner blood 1 h after injection, which coincident with early-peak Although late-peak responded antibody, no significant obtained before peak response. The found contain pyrogen activity, stable heating at 70 degrees C for 30 min resistant vitro treatment Rabbit also elicited rabbits, second phase be mediated similar pyrogen. These results suggest that plays important eliciting a febrile response endotoxin.