The multidrug ABC transporter BmrC/BmrD of Bacillus subtilis is regulated via a ribosome-mediated transcriptional attenuation mechanism

作者: Ewoud Reilman , Ruben A. T. Mars , Jan Maarten van Dijl , Emma L. Denham

DOI: 10.1093/NAR/GKU832

关键词: Cell biologyRepressorTranscription factorGene expressionBiologyGeneticsBacillus subtilisATP-binding cassette transporterOpen reading frameTranscriptional attenuationTranscriptional regulation

摘要: Expression of particular drug transporters in response to antibiotic pressure is a critical element the development bacterial multidrug resistance, and represents serious concern for human health. To obtain better understanding underlying regulatory mechanisms, we have dissected transcriptional activation ATP-binding cassette (ABC) transporter BmrC/BmrD Gram-positive model bacterium Bacillus subtilis. By using promoter-GFP fusions live cell array technology, demonstrate temporally controlled bmrCD genes antibiotics that target protein synthesis. Intriguingly, expression only occurs during late-exponential stationary growth stages, irrespective timing challenge. We show this due tight control by transition state regulator AbrB. Moreover, our results are co-transcribed with bmrB (yheJ), small open reading frame immediately upstream bmrC harbors three alternative stem-loop structures. These stem-loops apparently crucial antibiotic-induced transcription. Importantly, requires translation bmrB, which implies BmrB serves as leader peptide. Altogether, first time ribosome-mediated attenuation mechanism can ABC transporter.

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