Single-Channel Water Permeabilities of Escherichia coli Aquaporins AqpZ and GlpF
作者： Morten Ø. Jensen , Ole G. Mouritsen
DOI: 10.1529/BIOPHYSJ.105.073965
关键词: Selectivity filter 、 Lipid bilayer 、 Biophysics 、 Static electricity 、 Permeation 、 Crystallography 、 Escherichia coli 、 Osmosis 、 Chemistry 、 Permeability (electromagnetism) 、 Aquaporin
摘要: Abstract From equilibrium molecular dynamics simulations we have determined single-channel water permeabilities for Escherichia coli aquaporin Z (AqpZ) and aquaglyceroporin GlpF with the channels embedded in lipid bilayers. GlpF's osmotic permeability constant p f exceeds by 2–3 times that of AqpZ diffusive ( d ) is found to exceed 2–9-fold. Achieving complete selectivity consequently implies lower transport rates overall relative less selective, wider channel GlpF. For AqpZ, ratio / ≅12 close average number molecules lumen, whereas GlpF, ≅4. This single-file structure luminal more pronounced narrower two. Electrostatics profiles across pore lumens reveal significantly reinforces water-channel interactions, weaker water-water interactions turn suppress correlations Consequently, suppressed narrow filter become a key structural determinant permeation causing permeate slower AqpZ.
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