Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection
作者： Minjoung Jo , Ji-Young Ahn , Joohyung Lee , Seram Lee , Sun Woo Hong
关键词: Small molecule 、 SELEX Aptamer Technique 、 Combinatorial chemistry 、 Binding site 、 Biochip 、 Molecular biology 、 Biology 、 Oligonucleotide 、 Systematic evolution of ligands by exponential enrichment 、 Aptamer 、 Bisphenol A
摘要: The development of reagents with high affinity and specificity to small molecules is crucial for the high-throughput detection chemical compounds, such as toxicants or pollutants. Aptamers are short single-stranded (ss) oligonucleotides able recognize target affinity. Here, we report selection ssDNA aptamers that bind Bisphenol A (BPA), an environmental hormone. Using SELEX process, isolated BPA from a 1015 random library 60 mer ssDNAs. selected bound specifically BPA, but not structurally similar molecules, B one methyl group difference, 4,4′-Bisphenol 2 groups difference. these aptamers, developed aptamer-based sol–gel biochip detected dissolved in water. This novel can be further applied universal high-specificity molecules.
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