作者: Sizolwenkosi Mlotshwa , Zhiyong Yang , YunJu Kim , Xuemei Chen
DOI: 10.1007/S11103-006-0049-0
关键词: Petal 、 Cauliflower mosaic virus 、 Homeotic gene 、 Sepal 、 Nicotiana benthamiana 、 Arabidopsis 、 Wild type 、 Genetics 、 Biology 、 Mutant
摘要: Floral patterning and morphogenesis are controlled by many transcription factors including floral homeotic proteins, which organ identity is determined. Recent studies have uncovered widespread regulation of microRNAs (miRNAs), ~21-nucleotide non-coding RNAs that regulate protein-coding through transcript cleavage and/or translational inhibition. The the gene APETALA2 (AP2) miR172 crucial for normal Arabidopsis flower development likely to be conserved across plant species. Here we probe activity AP2/miR172 regulatory circuit in a heterologous Solanaceae species, Nicotiana benthamiana. We generated transgenic N. benthamiana lines expressing wild type AP2 (35S::AP2), miR172-resistant mutant (35S::AP2m3) MIR172a-1 (35S::MIR172) under control cauliflower mosaic virus 35S promoter. 35S::AP2m3 plants accumulated high levels mRNA protein exhibited defects included proliferation numerous petals, stamens carpels indicating loss determinacy. On other hand, nearly all 35S::AP2 barely detectable or were essentially non-phenotypic. Overall, data indicated expression ArabidopsisAP2 transgene was repressed at level an endogenous homologue could detected using probe. Interestingly, 35S::MIR172 had sepal-to-petal transformations more sepals suggesting interference with function perianth organs. Our uncover potential utility ArabidopsisAP2/miR172 system as tool manipulation architecture flowering time non-model plants.