DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein

摘要: DNA constructs are provided for stable transformation of plastids multicellular plants and expression foreign proteins in plastids. The comprise a transforming which is targeted to pre-determined location on the plastid genome inserted into by homologous recombination with targeting segments comprising sequences region genome. contains non-lethal selectable marker gene confers phenotype cells having substantially all genomes therein contain (i.e., homoplasmic or tissues). further comprises at least one insertion site 4 an additional segment, such as encoding protein improving characteristic transformed plant. preferably chimeric assembly from cassette 5' 3' regulatory segments, derived genes. A coding segment between form gene. preferred present invention aadA bacteria, encodes aminoglycoside 3"-adenylyltransferase confer spectinomycin streptomycin resistance.