Electrical therapies act on the Ca 2+ /CaM signaling pathway to enhance bone regeneration with bioactive glass [S53P4] and allogeneic grafts
作者： Gabriela B Chiarotto , Guilherme F Caetano , Glaucia M T Santos , Fernanda A S Mendonça , Camila A Oliveira
DOI: 10.1002/JBM.B.34858
关键词: Cell growth 、 Osteoblast 、 Cancer research 、 Bone regeneration 、 Bone remodeling 、 Viability assay 、 Bone sialoprotein 、 Calvaria 、 Materials science 、 Angiogenesis
摘要: This study aimed to investigate the application of low-intensity electrostimulation (ES) and electromagnetic stimulation (EM) associated with bioactive glass (BG) or allogeneic grafts (BB) in bone regeneration. A cell viability test on osteoblasts (UMR-106) was performed presence BB BG ES (10 μA/5 min) EM (500 Hz/2 min). Critical defects (25 mm2 ) calvaria were generated male Wistar rats, regeneration evaluated 30th, 60th, 120th days after surgery. Cell proliferation increased both BG. Bone remodeling more effective using graft therapies, angiogenesis, osteoblast proliferation, OPN expression + group. higher number osteoclasts, an increase sialoprotein, Runx-2, Opn gene found The therapy had Runx-2 Opn. Groups that numbers osteoblasts, expression. voltage-gated calcium channels groups ES, while calmodulin therapies without grafting. favored repair upon grafting by improving osteogenic expression, tissue reorganization. Despite activating different pathways, intracellular concentrations calmodulin, leading
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