AcidoCEST MRI Evaluates the Bone Microenvironment in Multiple Myeloma.

摘要: PURPOSE Multiple myeloma (MM) is an incurable disease of malignant plasma cells in the bone marrow (BM). Adaptive responses to hypoxia may be essential element MM progression and drug resistance. This metabolic adaptation involves a decrease extracellular pH (pHe), it depends on upregulation glucose transporters (GLUTs) that common cancer cells. CEST MRI imaging technique assesses pHe indirectly by exchange rate magnetic saturation transfer between labile protons solute water. Thus, this study aimed determine feasibility acidoCEST for measurement using orthotopic mouse model compared with GLUT1 immunofluorescence staining as reference. PROCEDURES Orthotopic BM engrafted xenografts were established NSG/NOD mice human RPMI8226 cell line. AcidoCEST was performed approximately 6 weeks after intravenous challenge, before administration iopamidol. values generated via fitting spectrum Bloch-McConnell equations. Samples decalcified, sectioned, immunostained expression. Pearson's correlation used assess relationship [H3O+] versus RESULTS Ten underwent followed immunofluorescent histologic analysis. A strong negative seen expression (r = - 0.75, p < 0.001). After transformation [H3O+], positive observed 0.8, CONCLUSIONS can measure affected multiple myeloma. In model, measured showed correlations phenotype tumor assessed histological assessment overexpression.