Proteomics Evidence for the Activity of the Putative Antibacterial Plant Alkaloid (-)-Roemerine: Mainstreaming Omics-Guided Drug Discovery

摘要: Abstract Discovery of new antibacterials with novel mechanisms is important to counteract the ingenious resistance bacteria. In this connection, omics-guided drug discovery offers a rigorous method in quest antibacterials. (-)-Roemerine plant alkaloid that has been reported possess putative antibacterial activity against Escherichia coli, Bacillus subtilis, and Salmonella typhimurium. The aim present study was characterize (-)-roemerine coli TB1 using proteomics tools. With treatment, we found limited permeability through outer membrane repression transport proteins involved carbohydrate metabolism, resulting poor carbon source availability. shortfall intracellular sources turn led impaired cell growth. reduction abundance related translational machinery, amino acid biosynthesis, metabolism accompanied by nutrient-limited state. latter finding...