Studies on the mechanism of Janus Green B staining of mitochondria: II. Reactions and properties of Janus Green B and its derivatives

摘要: Abstract 1. The absorption spectra of JG-B and its reduction products are reported. has spectrum maxima at 595, 390, 285 mμ. Diethylsafranine 555 272 Leucosafranine no maximum in the visible range. 2. Janus Green G (dimethylsafranineazodimethylaniline) 587, 395, 281 Dimethylsafranine 275 3. Two methods presented for determining relative amounts JG corresponding safranine derivative mixtures two dyes. 4. effect pH on stability derivatives were studied. 5. Whereas was readily reduced by cysteine glutathione, JG-G not significantly cysteine. 6. polarographic At 7.48 showed steps, one a potential −.001 volts, other −.188 volts. only step (E = −.214 volts).