Expanding the power of recombinase-based labeling to uncover cellular diversity

摘要: Investigating the developmental, structural and functional complexity of mammalian tissues organs depends on identifying gaining experimental access to diverse cell populations. Here, we describe a set recombinase-responsive fluorescent indicator alleles in mice that significantly extends our ability uncover cellular diversity by exploiting intrinsic genetic signatures uniquely define types. Using recombinase-based intersectional strategy, these new permit non-invasive labeling cells defined overlap up three distinct gene expression domains. In response different combinations Cre, Flp Dre recombinases, they express eGFP and/or tdTomato allow visualization full morphology. demonstrate value features through proof-of-principle analysis central noradrenergic system. We label previously inaccessible subpopulations neurons reveal details their three-dimensional architecture axon projection profiles. These will provide populations at unprecedented resolution, facilitating developmental origin anatomical, molecular physiological properties.