Iron and Pseudomonas aeruginosa biofilm formation

摘要: Iron serves as a signal in Pseudomonas aeruginosa biofilm development. We examined the influence of mutations known and putative iron acquisition-signaling genes on morphology. In iron-sufficient medium, mutants that cannot obtain through high-affinity pyoverdine acquisition system form thin biofilms similar to those formed by parent under low conditions. If an source for different is provided mutant, normal development occurs. This enabled us identify uptake gene clusters likely serve transport ferric citrate ferrioxamine. suggest functional P. active chelated or level internal iron. levels, then factor be involved signaling cytoplasmic regulator protein, Fur, which controls expression iron-responsive genes. support Fur involvement, we found with mutant was able organize into more mature than parent. The two Fur-controlled small regulatory RNAs (PrrF1 F2) do not appear mediate control information establishes mechanistic basis formation.