Molecular cloning of Bacillus subtilis genes involved in DNA metabolism.
作者： Marta Perego , Eugenio Ferrari , Maria Teresa Bassi , Alessandro Galizzi , Piergiorgio Mazza
DOI: 10.1007/BF00329829
关键词: Bacillus subtilis 、 DNA 、 Gene 、 Molecular cloning 、 Recombinant DNA 、 Plasmid 、 In vitro recombination 、 Biology 、 Restriction map 、 Molecular biology
摘要: Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 wild-type phenotype were isolated from library constructed in plasmid pJH101. A λ recombinant clone dnaC was obtained Charon 4A library. restriction map of the cloned fragments constructed. The 11.3 kb pMP60-13 containing sequence dnaG5 shown recF33 mutant as well.
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