Fine-structure genetic mapping of human chromosomes using the polymerase chain reaction on single sperm: experimental design considerations.

摘要: The polymerase chain reaction (PCR) makes it possible to rapidly generate a very large number of copies specific region DNA. Application PCR individual human sperm cells permits the typing independent male meiotic events. If donor is heterozygous at three loci, using will permit ordering loci in manner analogous classical methods experimental genetics. Sequential analysis trios by provide accurate means any tightly linked loci. Here, we describe design and sample-size issues raised application for mapping chromosomes, demonstrate that be an efficient method generating fine-structure genetic maps.