Structure of the sucrose synthase gene on chromosome 9 of Zea mays L

摘要: Abstract The structure of the shrunken gene Zea mays encoding sucrose synthase (EC 2.4.1.13) was determined by (i) sequencing transcription unit and ˜1.2 kb 5' -upstream sequences from a genomic clone, (ii) nearly full length cDNA clone (iii) determining start site combination primer extension experiments with synthetic oligodeoxynucleotide primers S1 mapping. The is 5.4 long, which 2746 bp are found in mature mRNA. interrupted 15 introns. first two introns ˜1 ˜0.5 length, respectively, while other much smaller. A TATA box located 30 upstream site. Approximately 610 direct repeat 16 nucleotides, separated 4-fold repetition sequence GGTGG detected. 16-bp has similarities to between promoters maize zein also expressed endosperm tissue. transposable element Ds mutant sh-m5933 sh-m6233 alleles inserted seventh intron, respectively. clones were obtained different lines. This allows determination polymorphic sites frequent 3rd codon position absent 1st 2nd positions. In addition, 3' -untranslated shows duplications that may have arisen insertion subsequent excision elements.