Antioxidant enzymes activities of Burkholderia spp. strains—oxidative responses to Ni toxicity

摘要: Increased agriculture production associated with intense application of herbicides, pesticides, and fungicides leads to soil contamination worldwide. Nickel (Ni), due its high mobility in soils groundwater, constitutes one the greatest problems terms environmental pollution. Metals, including Ni, concentrations are toxic cells by imposing a condition oxidative stress induction reactive oxygen species (ROS), which damage lipids, proteins, DNA. This study aimed characterize Ni antioxidant response two tolerant Burkholderia strains (one isolated from noncontaminated soil, SNMS32, other contaminated SCMS54), measuring superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), S-transferase (GST) activities. accumulation bacterial growth presence metal were also analyzed. The results showed that both exhibited different trends distinct enzymes responses. strain (SCMS54) higher biosorption an increase SOD GST activities after 5 12 h exposure. analysis SOD, CAT, GR nondenaturing PAGE revealed appearance extra isoenzyme SCMS54 for each enzyme. suggest present more plasticity potential be used water bioremediation.