Genetic Mapping of the Incompatibility Locus in Olive and Development of a Linked Sequence-Tagged Site Marker.

摘要: The genetic control of self-incompatibility (SI) has been recently disclosed in olive. Inter-varietal crossing confirmed the presence only two incompatibility groups (G1 and G2), suggesting a simple Mendelian inheritance trait. A ddRAD sequencing biparental population segregating for performed high-density linkage maps were constructed order to map SI locus identify gene candidates linked markers. progeny consisted full-sib family 229 individuals derived from cross ‘Leccino’ (G1) × ‘Dolce Agogia’ (G2) varieties, 1:1 (G1:G2), accordance with diallelic (DSI) model. total 16,743 single nucleotide polymorphisms was identified, 7,006 female parent 9,737 male Agogia’. Each parental 23 showed an unusual large size (5,680 cM 3,538 Agogia’). Recombination decreased across all pollen mother cells Agogia’, higher heterozygosity, compared megaspore ‘Leccino’, context species that exceptionally high recombination rates. subset 109 adult plants assigned either group by stigma test mapped interval 5.4 on 18. This region spanned approximately 300 Kb olive genome assembly. We developed Sequence-Tagged Site marker DSI identified five haplotypes 57 cultivars known assignment. combination SNPs sufficient predict G1 or G2 phenotypes cultivars, enabling early marker-assisted selection compatible genotypes allowing rapid screening inter-compatibility among guarantee effective fertilization increase production. construction led development first functional provided positional candidate genes locus.