Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum L.

摘要: Solanum nigrum is a newly discovered Cd-hyperaccumulator. In the present study, protective effects of proline against cadmium toxicity callus and regenerated shoots S. are investigated based on high frequency in vitro shoot regeneration system. Proline pretreatment reduces reactive oxygen species levels protects plasma membrane integrity under stress, therefore improves tolerance nigrum. Inductively coupled mass spectroscopy analysis shows that exogenous increases accumulation Further indicates improvement caused by correlated with an increase superoxide dismutase catalase activity intracellular total glutathione content. The interaction between enzymic or non-enzymic antioxidants discussed.