NUCLEAR AND CYTOPLASMIC ROLES IN DETERMINATION OF CELLULAR PHENOTYPE
作者： George E. Veomett
DOI: 10.1016/B978-0-12-518550-9.50049-4
关键词: Cell biology 、 Biology 、 Cytochalasin 、 Cytoplasm 、 Cytoplast 、 Extranuclear inheritance 、 Cellular component 、 Enzyme 、 Cellular phenotype 、 Fungal metabolite
摘要: Mammalian cells can be separated into enucleated (cytoplast) and nucleated (karyoplast) fragments by subjecting to a centrifugal force in the presence of fungal metabolite cytochalasin B. Cytoplasts karyoplasts have been partially characterized regarding their ultrastrueture, enzymatic constitution, physiological behavior. The isolated cellular components fused whole or each other. fusion cytoplasts provided first demonstration cytoplasmic inheritance mammalian cells. Viable are formed fusing
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