High activation and skewed T‐cell differentiation are associated with low IL‐17A levels in a hu‐PBL‐NSG‐SGM3 mouse model of HIV infection

摘要: The humanized NOD/SCID/IL-2 receptor γ-chainnull (NSG) mouse model has been widely used for the study of HIV pathogenesis. Here, NSG mice with transgenic expression human stem cell factor (SCF), granulocyte-macrophage colony-stimulating (GM-CSF) and interleukin (IL)-3 (NSG-SGM3) were injected peripheral blood leukocytes (PBL mice) from two HIV-infected (HIV+ ) patients who under anti-retroviral therapy (ART; referred as HIV+ or one HIV-seronegative healthy volunteer (HIV- ). Such are either hu-PBL-NSG-SGM3 HIV- mice, depending on source PBL. kinetics replication T responses following engraftment evaluated in secondary lymphoid tissues. High low CD4 : CD8 ratios observed absence (ART). Consistent high activation skewed differentiation cells donor, exhibited a higher co-expression leukocyte antigen D-related (HLA-DR) CD38 than well shifted to CCR7- CD45RA+ terminal effector profile, even presence ART. In addition, activation/differentiation disturbances associated decreased plasma levels IL-17A. Thus, this recapitulates some immune occurring patients, underlying its potential use studying pathogenic events during infection.