Honey Bee Hemocyte Profiling by Flow Cytometry

摘要: Multiple stress factors in honey bees are causing loss of bee colonies worldwide. Several infectious agents believed to contribute this problem. The mechanisms immunity not completely understood, part due limited information about the types and abundances hemocytes that help resist disease. Our study utilized flow cytometry microscopy examine populations hemolymph particulates bees. We found includes permeabilized cells, plasmatocytes, acellular objects resemble microparticles, listed order increasing abundance. cells plasmatocytes showed unexpected differences with respect properties plasma membrane labeling annexin V. Both failed show measurable mitochondrial potential by using JC-1 probe. results suggest particulate dynamic, revealing significant when comparing individual hive members, exposed diverse conditions. Shifts hemocyte likely represent changing conditions or metabolic colony members. A better understanding profiles may provide insight into physiological responses factors, some which be related failure.