Activation of mouse macrophages for tumor cell killing. I. Quantitative analysis of interactions between lymphokine and lipopolysaccharide.

摘要: Lymphokine-rich supernatants from concanavalin A-stimulated spleen cell cultures failed to activate mouse peritoneal macrophages for tumor killing, providing that the assay system was free of detectable (less than 0.125 ng/ml) endotoxin. Instead, increasing amounts lymphokine progressively increased sensitivity activation by purified bacterial lipopolysaccharide (LPS). Conversely, effect minute could be detected in presence relatively high (though nonactivating) concentrations LPS. The latter observation exploited develop a highly sensitive, quantitative lymphokine. In this assay, serially diluted were tested on macrophage monolayers constant concentration (3 Under these conditions, dose-response curve sigmoidal and therefore suitable conversion linear plot using logarithmic transformation von Krogh equation. Activity expressed quantitatively reproducibly terms units activity. By facilitating purification should contribute development more precise biochemical understanding role killing.