Investigation of Oxtr-expressing Neurons Projecting to Nucleus Accumbens using Oxtr-ires-Cre Knock-in prairie Voles (Microtus ochrogaster).
作者： Kengo Horie , Kiyoshi Inoue , Katsuhiko Nishimori , Larry J. Young
DOI: 10.1016/J.NEUROSCIENCE.2020.08.023
关键词: Basolateral amygdala 、 Anterior cingulate cortex 、 Prefrontal cortex 、 Prairie vole 、 Anterior olfactory nucleus 、 Oxytocin receptor 、 Biology 、 Neuroscience 、 Insular cortex 、 Nucleus accumbens
摘要: Social bonds such as parent-infant attachment or pair can be critical for mental and physical well-being. The monogamous prairie vole (Microtus ochrogaster) has proven useful examining the neural substrates regulating social behaviors, including bonding. Oxytocin (OXT) oxytocin receptor (OXTR) play roles in alloparental care, bonding consoling behavior voles. While OXTR a few regions, nucleus accumbnes (NAcc), prefrontal cortex (PFC) anterior cingulate (ACC), have been implicated these extent to which other OXT sensitive areas modulate behaviors not investigated. NAcc is central hub modulating dependent behaviors. To identify neurons expressing Oxtr brain, we generated gene knock-in voles Cre recombinase tandem with (Oxtr-ires-Cre) using CRISPR/Cas9 genome editing. We confirmed mRNA co-localization NAcc, validating this model. Next, identified putative Oxtr-expressing projecting by infusing retrograde CRE-dependent EGFP AAV into visualizing fluorescence. found enhanced green fluorescent protein (EGFP) positive olfactory nucleus, PFC, ACC, insular (IC), paraventricular thalamus (PVT), basolateral amygdala (BLA), posteromedial posterolateral cortical amygdaloid area (PMCo, PLCo). ACC projection may represent species-specific circuit since of mice were reported project NAcc. This first delineation circuits vole, demonstrates utility novel genetically modified organism characterizing involved
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