Diversity and characteristics of raw milk microbiota from Korean dairy farms using metagenomic and culturomic analysis

摘要: Abstract Bovine milk is highly nutritious, so it creates an ideal environment for the growth of microorganisms. Contamination with microorganisms contributes to overall bacterial composition and has a great influence on acidification deterioration milk. As dairy production consumption have increased over past decade, microbial gained attention. In Korea, products are rapidly gaining popularity, but studies in from Korean farms limited. present study, we performed metagenomic analysis high-throughput sequencing DNA identify bacteria raw collected 40 4 different provinces Korea: Gyeonggi-do, Chungcheong-do, Jeolla-do, Gyeongsang-do. Representative communities including Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria formed core microbiota at phylum level, Pseudomonas was found greatest provincial variation genus level. addition, determined prevalence spore-forming using two independent methods culturomic by MALDI-TOF next generation 16S rRNA genes, Bacillus thermoamylovorans identified account largest proportion. We further confirmed that B. able survive potentially toxigenic lab cheese model study Caenorhabditis elegans as simple host model. Our result clearly shows specific distinct proportions may cause spoilage, suggesting specified farm management practices depending region. These findings can help produce safe, high-quality develop new sanitation procedures well processing controls.