Effects of ageing on proliferative ability, and the expressions of secreted protein, acidic and rich in cysteine (SPARC) and osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human periodontal ligament cells.
作者： Hideki Shiba , Keiji Nakanishi , Masatoshi Sakata , Tsuyoshi Fujita , Yuushi Uchida
DOI: 10.1016/S0047-6374(00)00140-8
关键词: Biology 、 Osteoprotegerin 、 Cell growth 、 Internal medicine 、 In vitro 、 Osteonectin 、 Endocrinology 、 Ageing 、 Cell 、 In vivo 、 Osteoclast
摘要: Secreted protein, acidic and rich in cysteine (SPARC) has been suggested to play an important role wound repair mineralization. Osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF) is a secreted protein that inhibits the maturation, activity survival of osteoclasts. An examination changes expression these proteins as well their proliferative ability according ageing cultured cells may elucidate characteristic periodontal tissues induced by ageing. In present study, SPARC OPG/OCIF were compared between cultures human ligament (HPL) obtained from young senior donors (in vivo cellular ageing) HPL at early late passages vitro ageing). Cumulative population doubling levels cell time greater shorter, respectively, than those donors. Levels mRNA increased with vivo. No change was observed. The similar This study demonstrated for first relationship functional cells. These findings suggest impairment due decrease Furthermore, increase be related metabolism occur
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