In vitro affinity of 99Tcm-labelled N2S2 conjugates of chrysamine G for amyloid deposits of systemic amyloidosis.
作者： N. A. DEZUTTER , R. M. SCIOT , T. J. de GROOT , G. M. BORMANS , A. M. VERBRUGGEN
DOI: 10.1097/00006231-200105000-00014
关键词: Pathology 、 Congo red 、 Biopsy 、 In vivo 、 In vitro 、 Serum amyloid P component 、 Kidney 、 Amyloidosis 、 Amyloid 、 Chemistry
摘要: To date, systemic amyloidosis is diagnosed histologically in vitro using Congo red staining or vivo iodine-123 serum amyloid P component (123I-SAP) scintigraphy. 99Tcm-labelled derivatives of chrysamine G (CG), a lipophilic analogue red, were synthesized as potential tracer agents for direct and quantitative scintigraphic evaluation deposits. determine the affinity 99Tcm-MAMA-CG, 99Tcm-Me4MAMA-CG 99Tcm-MAMA-CG diethyl ester amyloid, autoradiography was performed on sections human kidney biopsy cylinders from kidneys with deposits (types AA, Alambda Akappa) control tissue after incubation respective agents. The binding its tetramethyl derivative higher to material Akappa type compared tissue. This prevented by presence 10 microM medium. 9Tcm-MAMA-CG did not demonstrate increased red-positive In conclusion, localize specifically tissue, suggesting that these may be applicable specific targeting diagnostic purposes clinical amyloidosis.
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