Tobramycin and bicarbonate synergise to kill planktonic Pseudomonas aeruginosa, but antagonise to promote biofilm survival.
作者： Karishma S Kaushik , Jake Stolhandske , Orrin Shindell , Hugh D Smyth , Vernita D Gordon
DOI: 10.1038/NPJBIOFILMS.2016.6
关键词: Antibiotic resistance 、 Antimicrobial 、 Microbiology 、 Aminoglycoside 、 Antibiotics 、 Bicarbonate 、 Biofilm 、 Tobramycin 、 Pseudomonas aeruginosa 、 Medicine
摘要: Increasing antibiotic resistance and the declining rate at which new antibiotics come into use create a need to increase efficacy of existing antibiotics. The aminoglycoside tobramycin is standard-of-care for many types Pseudomonas aeruginosa infections, including those in lungs cystic fibrosis (CF) patients. P. nosocomial opportunistic pathogen that, planktonic form, causes acute infections and, biofilm chronic infections. Inhaled bicarbonate has recently been proposed as therapy improve antimicrobial properties CF airway surface liquid viscosity mucus. Here we measure effect combining against aeruginosa, both lab strains clinical isolates. Bicarbonate synergises with enhance killing bacteria. In contrast, antagonises promote better growth. This suggests caution when evaluating infected biofilms. We analyse interactions using an interpolated methodology dose-response function. These surfaces allow more accurate estimation combinations yielding synergy antagonism than do standard isobolograms. By incorporating predictions based on Loewe additivity theory, can consolidate information wide range that produce complex surface, single number measures net effect. tool thus allows rapid initial potential benefit or harm therapeutic combination. Software code freely made available resource community.
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