Studies of rhizobial competitiveness for nodulation in soybean using a non-destructive split-root system

摘要: Split-root systems (SRS) constitute an appropriate methodology for studying the relevance of both local and systemic mechanisms that participate in control rhizobia-legume symbioses. In fact, this kind approach allowed to demonstrate autoregulation nodulation (AON) response soybean 1980s. SRS, plant main root is cut two lateral roots emerge from seedlings after root-tip removal are confined into separate compartments. After several days growth, these plants have can be inoculated with same or different bacteria, at times. work, we used a non-destructive SRS study competitiveness relations between rhizobial strains roots. One them competition soybean-nodulating rhizobia: slow-grower Bradyrhizobium japonicum USDA110 fast-grower Sinorhizobium fredii HH103. The second competitive blocking S. 257DH4 American Osumi by USDA257, which unable nodulate soybeans. Our results showed relationships studied work mitigated even avoided when spatially separated compartments containing half-roots plant, suggesting more related responses. our opinion, split-root further among strains.