Biological Screening of Organotin(IV) Complexes

摘要: Metal complexes generally and organotin(IV) compounds especially are used routinely in several biomedical commercial applications like agricultural biocides, disinfectants, antitumor agents, wood preservatives, antioxidants, stabilizers for polyvinylchloride, marine antifouling coating, anti-herpes flame retardants, smoke suppressants, anti-wear homogenous catalysts recycling agents. A number of organotin have been shown to be toxic, there is increasing concern regarding their widespread use in environmental biological systems. Among compounds, organotin carboxylates highly important; these contain Sn bond formed through COO¯ group a number interesting structural features applications. Three groups novel including mono-, di- and triderivatives ligands: 3,4-methylenedioxy 6-nitrophenylpropenoic acid (L1), 3,4- methylenedioxyphenylpropenoic acid (L2) 2,3-methylenedioxybenzoic (L3) were examined their different activities. In vitro antimicrobial activities determined against six pathogenic bacterial strains (antibacterial assay) six fungal (antifungal assay). Determined by the antimicrobial studies demonstrated higher level activity towards all tested strains than respective free ligands. As whole, antibacterial activities decreased order of; diorganotin tri-organotin carboxylates mono-organotin ligand acids. However, tri-organotin best antifungal activities. Since inhibited growth micro-organisms, it has assumed that production an enzyme affected, hence micro-organisms less able to metabolize nutrients consequently, ceased. All also investigated cytotoxicity crown gall tumor inhibition (potato disc) assay brine-shrimp lethality bioassay, respectively. determined brineshrimp lethality assay; or cytotoxic compounds fall di-organotin carboxylates, similar pattern is reported literature. Such may yield new leads development drugs, which can display different spectrum activity. A panel tri-organotin(IV) that higher previous assays selected investigate effect on MCF-7 cells. the clonogenic assay, (0.1 µg/ml, 1.0 µg/ml or 10.0 µg/ml) markedly following treatment cells with particularly carboxylates. Further investigation was aimed determine genotoxic biochemicalcell fingerprint cells (0.01 0.1 assay. Induction DNA single-strand breaks (SSBs) measured using alkaline single-cell gel electrophoresis (comet) relative micronucleus-forming using cytokinesis-block micronucleus (CBMN) as well alterations biochemical-cell signatures ascertained attenuated total reflection fourier transform infrared (ATR-FTIR) spectroscopy. In comet analysis, although marked fluctuations background levels SSBs measurable control cell populations, remarkable consistency ability organotin(IV) comet-forming noted. with more difficult distinguish between abilities induce elevations SSBs. Probably consequence lower relative cytotoxicities, observed either mono- di-organotins compared tri-organotins. Following interrogation of treated-cell populations ATR-FTIR spectroscopy, spectra (900 1800 cm-1) derived from organotin-treated exhibited clear biochemical-cell exposures low 0.0001 µg/ml; this evident DNA/RNA spectral region ( 1490 1000 cm-1). These results differential biocidal, cytotoxic, effects compounds.