Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum

摘要: True cellulase activity has been demonstrated in cell-free preparations from the thermophilic anaerobe Clostridium thermocellum. Such depends upon presence of Ca and a thiol-reducing agent which dithiothreitol is most promising. Under these conditions, native (cotton) derived forms cellulose (Avicel filter paper) were all extensively solubilized at rates comparable with Trichoderma reesei. Maximum was displayed 70 degrees C pH 5.7 6.1 on Avicel carboxymethylcellulose, respectively. In absence substrate temperatures up to C, carboxymethylcellulase much more unstable than Avicel-hydrolyzing activity.