“FISHing” to detect urinary and other cancers

摘要: BACKGROUND: The UroVysion Bladder Cancer Kit detects amplifications of chromosomes 3, 7, and 17, the deletion 9p21, by fluorescence in situ hybridization (FISH). Because manual interpretation FISH is time consuming can be challenged variable probe signal strengths background labeling, authors investigated an automated image analysis system to improve throughput, productivity, quality control, accuracy. METHODS: The evaluated interactive BioView Duet imaging as aid a 2-armed, blinded comparison with screens same 135 consecutive cases. Manual Duet-assisted interpretations were compared respect concordance, reproducibility, timing. RESULTS: Eighty-one cases interpreted positive or negative 94% concordance kappa value 0.84 between Duet-aided interpretations. Three that ultimately judged detected but missed screen. A final could not given for≈25% Duet-scanned was highly reproducible for patient control slides. Pathologist evaluation per case 4 minutes 30 interpretation. Cytotechnologist involvement added 18 total 22 minutes, savings 8 case. CONCLUSIONS: Duet-aided at least equivalent The permitted review abnormal cells had ability evaluate brightfield cytology followed FISH. processing tools enhanced morphology skills professionals providing accurate (Cancer Cytopathol) 2010. © 2010 American Society.