Determination of citalopram in fish brain tissue: benefits of coupling laser diode thermal desorption with low- and high-resolution mass spectrometry.

摘要: Recent state-of-the-art methods developed for the analysis of polar xenobiotics from different types biological matrices usually employ liquid chromatography with mass spectrometry. However, there are limitations when a small amount sample is available. For example, individual benthic invertebrates or fish tissue samples often weigh less than 100 mg (e.g., brain, liver) but necessary to understand environmental fate and bioaccumulation dynamics. We ultra-fast based on direct introduction technique. This included coupling laser diode thermal desorption atmospheric pressure chemical ionization spectrometry (LDTD-APCI-MS). then quantitated common selective serotonin reuptake inhibitor (citalopram) in brain tissues juvenile after vivo exposure environmentally relevant concentration. Two spectrometric low (LDTD-APCI-triple quadrupole (QqQ)-MS/MS) high (LDTD-APCI-high-resolution product scan (HRPS)) resolutions were evaluated. Individual instrument conditions optimized achieve an accurate robust analytical method minimum preparation requirements. achieved very good recovery (97-108%) across range 1-100 ng g-1 LDTD-APCI-HRPS. LDTD-APCI-QqQ-MS/MS showed poorer performance due interferences matrix at lowest concentration level. LDTD-APCI was successfully validated non-filtered extracts. Evaluation final performed set real samples, including comparison LDTD-APCI-HRPS previously LC-heated electrospray ionization-HRPS method. new avoids chromatographic step provides important benefits such as limited masses, lower total volume (typically μL), reduction time per run few seconds. Graphical abstract.