Bronchoalveolar lavage in an animal model of acute lung injury: relationship between enhanced membrane permeability and transvascular neutrophil flux

摘要: Results of studies utilizing bronchoalveolar lavage (BAL) have led workers to propose that the neutrophil serves as pivotal cellular element responsible for promoting enhanced alveolar capillary membrane (ACM) permeability in certain forms acute lung injury. The authors performed BAL on anesthetized, intubated, instrumented sheep before and after administration 15 mg/kg ethchlorvynol, a known pulmonary edemagenic agent. Bronchoalveolar fluid (BALF) protein content increased from 0.62 +/- 0.05 1.5 0.15 mg/ml, percentage neutrophils recovered 2% 1% at baseline 35% 7% (P less than 0.01) 60 minutes infusion ethchlorvynol. After ethchlorvynol into neutropenic (less 500 cells/microliter), BALF 0.35 0.08 0.69 mg/ml with no increase count. In 3 non-neutropenic was 30 infusion. significantly within minutes, whereas did not change. These findings suggest coexistent ACM injury reflected by increases number does necessarily imply cause-and-effect relationship