Cardiac Nitric Oxide Synthases and Na⁺/K⁺-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone.
作者： Snežana Tepavčević , DV Milutinović , D Macut , Jelena Stanišić , M Nikolić
关键词: Polycystic ovary 、 Dihydrotestosterone 、 Endothelial NOS 、 Nitric oxide synthase 、 Biology 、 Nitric oxide 、 Enos 、 Na+/K+-ATPase 、 Endocrinology 、 ATPase 、 Internal medicine
摘要: Abstract ▼ Nitric oxide synthases (NOSs) and Na + /K -ATPase are enzymes essential for regular functioning of the heart. Since both under insulin androgen regulation since action level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric (NO) production sodium/potassium transport would be deteriorated PCOS. To test our hypothesis introduced ani-mal model PCOS based on dihydrotestosterone (DHT) treatment female Wistar rats ana-lyzed protein expression, phosphorylation or sub-cellular localization endothelial NOS (eNOS), inducible (iNOS) alpha subunits Obtained results indi -cate DHT significantly decreased eNOS activating phos-phorylation at serine 1 177, while inhibitory phos -phorylation threonine 495 was increased. In contrast to expression eNOS, iNOS heart DHT-treated elevated. Furthermore, subunit ATPase, as well its plasma membrane content, with line this, 2 fraction membranes also below control level. conclusion, impaired effectiveness NOSs
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