Telomere susceptibility to cigarette smoke-induced oxidative damage and chromosomal instability of mouse embryos in vitro

摘要: Cigarette smoke is associated with high risk of lung, cardiovascular, and degenerative diseases, reduced fertility, possibly the health newborns. contains many components exerts its genotoxicity in part by generating reactive oxidative stress. Telomeres consist repeated 'G' rich sequences proteins located at chromosomal ends that maintain integrity. We tested hypothesis telomere shortening dysfunction are implicated damage instability using early mouse embryos vitro short-telomere model. Mouse exposed to components, cigarette condensate (CSC) concentration 0.02 mg/ml continuously or 0.1mg/ml for 20 h, cadmium 5-100 microM, exhibited increased stress loss, instability, apoptosis, compromised embryo cleavage development. Remarkably, reduction an antioxidant N-acetyl-L-cysteine (NAC) greatly these toxicities. Notably, led more severe dysfunction, which could be effectively rescued treatment, than did CSC. Moreover, short telomeres predisposed component-induced damage. These data further extend our understanding mechanisms underlying smoke-induced include instability.