Viperin catalyzes methionine oxidation to promote protein expression and function of helicases

摘要: Helicases play pivotal roles in fundamental biological processes, and posttranslational modifications regulate the localization, function, stability of helicases. Here, we report that methionine oxidation representative helicases, including DNA RNA helicases viral (ORF44 KSHV) cellular (MCM7 RIG-I) origin, promotes their expression functions. Cellular viperin, a major antiviral interferon-stimulated gene whose functions beyond host defense remain largely unknown, catalyzes these Moreover, biochemical studies entailing loss-of-function mutations pharmacological inhibitor interfering with lipid metabolism and, hence, decreasing viperin activity indicate potently increases enzyme are critical for replication immune activation. Our work uncovers role catalyzing implicated diverse processes.