The Transposon Galileo Generates Natural Chromosomal Inversions in Drosophila by Ectopic Recombination

摘要: Background Transposable elements (TEs) are responsible for the generation of chromosomal inversions in several groups organisms. However, Drosophila and other Dipterans, where abundant both as intraspecific polymorphisms interspecific fixed differences, evidence a role TEs is scarce. Previous work revealed that transposon Galileo was involved two polymorphic buzzatii. Methodology/Principal Findings To assess impact evolution shed light on mechanism involved, we isolated sequenced breakpoints another widespread inversion from D. buzzatii, 2z3. In non inverted chromosome, 2z3 distal breakpoint located between genes CG2046 CG10326 whereas proximal lies novel have named Dlh Mdp. analysis sequences relatively large insertions (2,870-bp 4,786-bp long) including copies (subfamily Newton), one at each breakpoint, plus TEs. The copies: (i) inserted opposite orientation; (ii) present exchanged target site duplications; (iii) chimeric. Conclusions/Significance Our observations provide best gathered so far inversions. addition, they show unequivocally ectopic recombination causative mechanism. fact three buzzatii investigated were generated by same family remarkable conceivably due to Galileo's unusual structure current (or recent) transpositional activity.