Egg Coat Proteins Activate Calcium Entry into Mouse Sperm via CATSPER Channels
作者： Jingsheng Xia , Dejian Ren
DOI: 10.1095/BIOLREPROD.108.074039
关键词: Immunology 、 Sperm motility 、 Biology 、 Cell biology 、 Zona pellucida 、 Acrosome reaction 、 Sperm 、 Voltage-dependent calcium channel 、 Patch clamp 、 Human fertilization 、 Egg protein
摘要: During mammalian fertilization, the contact between sperm and egg triggers increases in intracellular Ca2+ concentration ([Ca2++]i) sperm. Voltage-gated channels (CaVs) are believed to mediate initial phase of [Ca2+]i induced by coat (zona pellucida [ZP]) glycoproteins, while store depletion-activated entry is thought sustained phase. Using patch-clamp recording imaging, we show herein that CaV channel currents, found spermatogenic cells, not detectable epididymal essential for ZP-induced changes. Instead, CATSPER localized distal portion (the principal piece) required increases. Furthermore, increase starts from tail propagates toward head.
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