Sensitive Leptospira DNA detection using tapered optical fiber sensor.
作者： Nurul H Zainuddin , Hui Y Chee , Muhammad Z Ahmad , Mohd A Mahdi , Muhammad H Abu Bakar
关键词: Triethoxysilane 、 Leptospira DNA 、 DNA 、 Complementary DNA 、 Optoelectronics 、 Single-mode optical fiber 、 Fiber optic sensor 、 Materials science 、 Wavelength 、 Glutaraldehyde
摘要: This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The causes Leptospirosis, deadly disease but with common early flu-like symptoms. Optical single mode (SMF) 125 μm diameter is produce 12 waist and 15 cm length. novel DNA-based functionalized by incubating region sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane glutaraldehyde. Probe DNA immobilized onto subsequently hybridized its complementary (cDNA). transmission spectra are measured in 1500 1600 nm wavelength range. It discovered that shift SMF linearly proportional increase cDNA concentrations from 0.1 1.0 nM. sensitivity toward be 1.2862 nm/nM able as low fM. indicates high specificity when only minimal detected for non-cDNA testing. developed distinguish between actual serovars (Canicola Copenhageni) against Clostridium difficile (control sample) at very (femtomolar) target concentrations.
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