Chromosome homology and heterochromatin in goat, sheep and ox studied by banding techniques

作者: H. J. Evans , R. A. Buckland , A. T. Sumner

DOI: 10.1007/BF00399407

关键词: BiologyCentromereG bandingSatellite DNAGiemsa stainChromosomeGeneticsAutosomeHeterochromatinMetaphase

摘要: Peripheral blood lymphocyte metaphase chromosomes of three Bovoidean species have been studied using Quinacrine fluorescence and Giemsa banding techniques to give Q-, G-, C-banding patterns. Q- G-banding characteristics, coupled with chromosome length, enabled all the in each complements be clearly distinguished, although some difficulties were encountered very smallest chromosomes. A comparison patterns between revealed a remarkable degree homology Each 23 different acrocentric autosomes domestic sheep (2n=54) was represented by an identical goat (2n=60) arms 3 pairs metacentric matches remaining 6 acrocentrics. similar interspecies evident for but two ox (2n=60). This elements confirms previously suggested Robertsonian variation. The close these related indicates that are evolutionarily conservative may useful guide assessing interspecific relationships. —The centromeric heterochromatin found show little or no Q-or G-staining, contrast sex lack staining G-technique (ASG) contrasts markedly results obtained other mammalian species. However, technique regions normal intense stain C-bands inconspicuous. amount is considerable less than newly derived element discovered goat. It pale G-staining might presence G-Crich satellite DNA.

参考文章(38)
M. E. Drets, M. W. Shaw, Specific Banding Patterns of Human Chromosomes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 68, pp. 2073- 2077 ,(1971) , 10.1073/PNAS.68.9.2073
R.A. Buckland, H.J. Evans, A.T. Sumner, Identifying mouse chromosomes with the ASG technique. Experimental Cell Research. ,vol. 69, pp. 231- 236 ,(1971) , 10.1016/0014-4827(71)90333-8
M. L. Pardue, J. G. Gall, Chromosomal Localization of Mouse Satellite DNA Science. ,vol. 168, pp. 1356- 1358 ,(1970) , 10.1126/SCIENCE.168.3937.1356
W YASMINEH, J YUNIS, Satellite DNA in calf heterochromatin Experimental Cell Research. ,vol. 64, pp. 41- 48 ,(1971) , 10.1016/0014-4827(71)90190-X
N.O. Bianchi, J. Ayres, Polymorphic patterns of heterochromatin distribution in guinea pig chromosomes. Chromosoma. ,vol. 34, pp. 254- 260 ,(1971) , 10.1007/BF00286151
L. Zech, E.P. Evans, C.E. Ford, A. Gropp, Banding patterns in mitotic chromosomes of tobacco mouse Experimental Cell Research. ,vol. 70, pp. 263- 268 ,(1972) , 10.1016/0014-4827(72)90213-3
DAVID E. COMINGS, EVANGELITA AVELINO, DNA Loss during Robertsonian Fusion in Studies of the Tobacco Mouse Nature. ,vol. 237, pp. 199- 199 ,(1972) , 10.1038/NEWBIO237199A0
M. L. O'RIORDAN, J. A. ROBINSON, K. E. BUCKTON, H. J. EVANS, Distinguishing between the Chromosomes involved in Down's Syndrome (Trisomy 21) and Chronic Myeloid Leukaemia ( Ph 1 ) by Fluorescence Nature. ,vol. 230, pp. 167- 168 ,(1971) , 10.1038/230167A0