Heavy metals and human spermatozoa. III. The toxicity of copper ions for spermatozoa.

摘要: The dissolution of copper ions from metal into a saline medium in vitro was quantified using colourimetric assay. presence spermatozoa enhanced this and increasing the protein content further increased rate dissolution. Approximately 17% released either tightly bound to or within cell could not be removed by repeated washing. Once were immobilized, they revived washing changes medium, addition specific-chelating agent extensive dialysis. When toxicity cuprous cupric compared with metal, it shown that quantity required (0.2-0.4 mg/ml) excess total solution. ion (0.08-0.16 exert similar toxic effects copper, range metal. Under conditions study, is possible would oxidised form generating free radicals process. It known whether effect due ion, per se, generated its oxidation. Increasing levels low (8 high (64 values reported human uterine fluid but also offered some protection against ions.