Changes induced in the permeability barrier of the yeast plasma membrane by cupric ion.
作者： Y Ohsumi , K Kitamoto , Y Anraku
DOI: 10.1128/JB.170.6.2676-2682.1988
关键词: Biochemistry 、 Permeability (electromagnetism) 、 Biology 、 Amino acid 、 Vacuolar transport 、 Divalent 、 Membrane potential 、 Membrane 、 Vacuole 、 Plasma membrane Ca2+ ATPase
摘要: A specific effect of Cu2+ eliciting selective changes in the permeability intact Saccharomyces cerevisiae cells is described. When 100 microM CuCl2 was added to a cell suspension buffer low ionic strength, barrier plasma membranes lost within 2 min at 25 degrees C. The release amino acids partial, and composition released different from that those retained cells. Mostly glutamate released, but arginine mainly Cellular K+ rapidly after addition, 30% total These other observations suggested caused lesions membrane did not affect vacuolar membrane. were induced by divalent cations tested. novel simple method for differential extraction cytosolic acid pools treatment established. Ca2+ Cu2+-treated cells, large amount sequestered into vacuoles, with formation an inclusion Ca2+-polyphosphate complex vacuoles. also showed enhanced uptake basic S-adenosylmethionine. transport these substrates saturable kinetics affinities, reflecting process situ. With treatment, leakage compartment appears create concentration gradient across generates inside-negative potential, which may provide driving force positively charged substances provides useful situ investigating mechanisms solute pool phenomena
core.ac.uk
sci-hub.se 参考文章(19)
E Uchida, Y Ohsumi, Y Anraku, Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 260, pp. 1090- 1095 ,(1985) , 10.1016/S0021-9258(20)71211-1
T Sato, Y Ohsumi, Y Anraku, An arginine/histidine exchange transport system in vacuolar-membrane vesicles of Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 259, pp. 11509- 11511 ,(1984) , 10.1016/S0021-9258(18)90891-4
Y. Ohsumi, Y. Anraku, Active transport of basic amino acids driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 256, pp. 2079- 2082 ,(1981) , 10.1016/S0021-9258(19)69736-X
J.J. Gits, M. Grenson, Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. II. Evidence for a specific lysine-transporting system. Biochimica et Biophysica Acta. ,vol. 127, pp. 507- 516 ,(1966) , 10.1016/0005-2736(67)90040-5
Y Wada, Y Ohsumi, M Tanifuji, M Kasai, Y Anraku, Vacuolar ion channel of the yeast, Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 262, pp. 17260- 17263 ,(1987) , 10.1016/S0021-9258(18)45370-7
J. T. Murphy, K. D. Spence, Transport of S-Adenosylmethionine in Saccharomyces cerevisiae Journal of Bacteriology. ,vol. 109, pp. 499- 504 ,(1972) , 10.1128/JB.109.2.499-504.1972
T Sato, Y Ohsumi, Y Anraku, Substrate specificities of active transport systems for amino acids in vacuolar-membrane vesicles of Saccharomyces cerevisiae. Evidence of seven independent proton/amino acid antiport systems. Journal of Biological Chemistry. ,vol. 259, pp. 11505- 11508 ,(1984) , 10.1016/S0021-9258(18)90890-2
Pilar PENA, Francisco BARROS, Santiago GASCoN, Sofia RAMOS, Pedro S. LAZO, The Electrochemical Proton Gradient of Saccharomyces The Role of Potassium FEBS Journal. ,vol. 123, pp. 447- 453 ,(1982) , 10.1111/J.1432-1033.1982.TB19788.X
Y Ohsumi, Y Anraku, Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 258, pp. 5614- 5617 ,(1983) , 10.1016/S0021-9258(20)81935-8
L A Okorokov, L P Lichko, I S Kulaev, Vacuoles: main compartments of potassium, magnesium, and phosphate ions in Saccharomyces carlsbergenis cells. Journal of Bacteriology. ,vol. 144, pp. 661- 665 ,(1980) , 10.1128/JB.144.2.661-665.1980