Mathematical treatment of electrophoretically mediated microanalysis.

摘要: A new concept in reaction-based chemical analysis is introduced and theoretically described. By utilization of the variability electrophoretic mobilities among charged species, spatially distinct zones reagents can be electrophoretically merged under influence an applied electric field. Electrophoretically mediated microanalysis (EMMA) exploits this phenomenon as a basis for utilizing capillary systems. EMMA described terms four stages required analysis: (1) analyte analytical reagent metering; (2) initiation reaction; (3) control reaction conditions product formation; (4) detection species whose production or depletion indicative concentration quantity interest. The method illustrated by enzymatic oxidation ethanol to acetaldehyde alcohol dehydrogenase with concurrent reduction NAD+ NADH monitored at 340 nm. Experimental results both substrate enzyme determinations are shown agree presented theory.