Molecular cloning, purification, and characterization of a novel polyMG-specific alginate lyase responsible for alginate MG block degradation in Stenotrophomas maltophilia KJ-2

作者: Su In Lee , Sung Hee Choi , Eun Yeol Lee , Hee Sook Kim

DOI: 10.1007/S00253-012-4266-Y

关键词: HydrolysisActive siteGlycosidic bondBiologyRecombinant DNAChondroitin B LyaseAmino acidMolecular cloningBiochemistryNucleotide

摘要: … alginate degradation. G red-β reducing end signals (4.90–4.86 ppm) appeared after alginate degradation by polyG-specific alginate … after alginate degradation by KJ-2 alginate lyase. …

参考文章(34)
Molecular identification of fungi. Molecular identification of fungi.. ,(2010) , 10.1007/978-3-642-05042-8
Arthur Weissbach, Jerard Hurwitz, The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. Journal of Biological Chemistry. ,vol. 234, pp. 705- 709 ,(1959) , 10.1016/S0021-9258(18)70158-0
Helga Ertesvåg, Frode Erlien, Gudmund Skjåk-Bræk, Bernd H. A. Rehm, Svein Valla, Biochemical Properties and Substrate Specificities of a Recombinantly Produced Azotobacter vinelandii Alginate Lyase Journal of Bacteriology. ,vol. 180, pp. 3779- 3784 ,(1998) , 10.1128/JB.180.15.3779-3784.1998
AM Ciabarra, JM Sullivan, LG Gahn, G Pecht, S Heinemann, KA Sevarino, Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family The Journal of Neuroscience. ,vol. 15, pp. 6498- 6508 ,(1995) , 10.1523/JNEUROSCI.15-10-06498.1995
Hee Sook Kim, Choul-Gyun Lee, Eun Yeol Lee, Alginate lyase: Structure, property, and application Biotechnology and Bioprocess Engineering. ,vol. 16, pp. 843- 851 ,(2011) , 10.1007/S12257-011-0352-8
Franceses Clementi, Alginate Production by Azotobacter Vinelandii Critical Reviews in Biotechnology. ,vol. 17, pp. 327- 361 ,(1997) , 10.3109/07388559709146618
Jae-Hwa Lee, In Su Lee, Eun Yeol Lee, Jung Won Shin, Sung Hee Choi, Dong Eun Kim, Hee Sook Kim, Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes Bioprocess and Biosystems Engineering. ,vol. 34, pp. 113- 119 ,(2011) , 10.1007/S00449-010-0452-4
A. J. Wargacki, E. Leonard, M. N. Win, D. D. Regitsky, C. N. S. Santos, P. B. Kim, S. R. Cooper, R. M. Raisner, A. Herman, A. B. Sivitz, A. Lakshmanaswamy, Y. Kashiyama, D. Baker, Y. Yoshikuni, An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae Science. ,vol. 335, pp. 308- 313 ,(2012) , 10.1126/SCIENCE.1214547
Masayuki Yamasaki, Satoko Moriwaki, Osamu Miyake, Wataru Hashimoto, Kousaku Murata, Bunzo Mikami, Structure and Function of a Hypothetical Pseudomonas aeruginosa Protein PA1167 Classified into Family PL-7 A NOVEL ALGINATE LYASE WITH A β-SANDWICH FOLD Journal of Biological Chemistry. ,vol. 279, pp. 31863- 31872 ,(2004) , 10.1074/JBC.M402466200