Glycerophosphatidylcholine PC(36:1) absence and 3′-phosphoadenylate (pAp) accumulation are hallmarks of the human glioma metabolome
作者： Wenchen Li , Hongmei Jia , Qi Li , Jiayue Cui , Ri Li
DOI: 10.1038/S41598-018-32847-8
关键词: Glioma 、 Chemistry 、 Regulation of gene expression 、 Metabolomics 、 Metabolome 、 Nucleotidase 、 Gene expression 、 Inositol monophosphatase 、 Metabolic pathway 、 Cancer research
摘要: Glioma is the most prevalent malignant brain tumor. A comprehensive analysis of glioma metabolome still lacking. This study aims to explore new special metabolites in tissues. non-targeted human metabolomics was performed by UPLC-Q-TOF/MS. The gene expressions 18 enzymes associated with 3’-phosphoadenylate (pAp) metabolism examined qRT-PCR. Those cover primary metabolic pathway pAp. We identified 15 (13 lipids and 2 nucleotides) that were significantly different between control Glycerophosphatidylcholine [PC(36:1)] content high pAp low (p < 0.01). In tissues, PC(36:1) not detected increased. 3′-nucleotidases (Inositol monophosphatase (IMPAD-1) 3′(2′),5′-bisphosphate nucleotidase 1(BPNT-1)) dramatically down-regulated. Meanwhile, expression 8 sulfotransferases (SULT), phosphoadenosine phosphosulfate synthases (PAPSS-1 PAPSS-2) L-aminoadipate-semialdehyde dehydrogenase-phosphopante-theinyl transferase (AASDHPPT) up-regulated. absence accumulation are noticeable aberration glioma. dramatic down-regulation IMPAD-1 BPNT-1 cause for accumulation. Our findings suggest differential discovered could be used as potentially novel therapeutic targets or diagnostic biomarkers abnormal nucleotides play roles pathogenesis
nature.com
harvard.edu
sci-hub.se 参考文章(48)
Jonas Dehairs, Rita Derua, Natalia Rueda-Rincon, Johannes V. Swinnen, Lipidomics in drug development. Drug Discovery Today: Technologies. ,vol. 13, pp. 33- 38 ,(2015) , 10.1016/J.DDTEC.2015.03.002
Sriram Venneti, Craig B. Thompson, Metabolic modulation of epigenetics in gliomas. Brain Pathology. ,vol. 23, pp. 217- 221 ,(2013) , 10.1111/BPA.12022
Ada W. Y. Leung, Wieslawa H. Dragowska, Daniel Ricaurte, Brian Kwok, Veena Mathew, Jeroen Roosendaal, Amith Ahluwalia, Corinna Warburton, Janessa J. Laskin, Peter C. Stirling, Mohammed A. Qadir, Marcel B. Bally, 3’-Phosphoadenosine 5’-phosphosulfate synthase 1 (PAPSS1) knockdown sensitizes non-small cell lung cancer cells to DNA damaging agents Oncotarget. ,vol. 6, pp. 17161- 17177 ,(2015) , 10.18632/ONCOTARGET.3635
Matthew S. Waitkus, Bill H. Diplas, Hai Yan, Isocitrate dehydrogenase mutations in gliomas. Neuro-oncology. ,vol. 18, pp. 16- 26 ,(2016) , 10.1093/NEUONC/NOV136
Jianguo Xia, Igor V. Sinelnikov, Beomsoo Han, David S. Wishart, MetaboAnalyst 3.0—making metabolomics more meaningful Nucleic Acids Research. ,vol. 43, ,(2015) , 10.1093/NAR/GKV380
R. Caivano, A. Lotumolo, P. Rabasco, A. Zandolino, F. D'Antuono, A. Villonio, M. I. Lancellotti, L. Macarini, A. Cammarota, 3 Tesla magnetic resonance spectroscopy: cerebral gliomas vs. metastatic brain tumors. Our experience and review of the literature International Journal of Neuroscience. ,vol. 123, pp. 537- 543 ,(2013) , 10.3109/00207454.2013.774395
Eleonora Seelig, Ulrich Keller, Markus Klarhöfer, Klaus Scheffler, Serge Brand, Edith Holsboer-Trachsler, Martin Hatzinger, Stefan Bilz, Neuroendocrine Regulation and Metabolism of Glucose and Lipids in Primary Chronic Insomnia: A Prospective Case-Control Study PLoS ONE. ,vol. 8, pp. e61780- 8 ,(2013) , 10.1371/JOURNAL.PONE.0061780
Elke Hattingen, Oliver Bähr, Johannes Rieger, Stella Blasel, Joachim Steinbach, Ulrich Pilatus, Phospholipid metabolites in recurrent glioblastoma: in vivo markers detect different tumor phenotypes before and under antiangiogenic therapy. PLOS ONE. ,vol. 8, ,(2013) , 10.1371/JOURNAL.PONE.0056439
Li Pan, Yunping Qiu, Tianlu Chen, Jinchao Lin, Yi Chi, Mingming Su, Aihua Zhao, Wei Jia, An optimized procedure for metabonomic analysis of rat liver tissue using gas chromatography/time-of-flight mass spectrometry Journal of Pharmaceutical and Biomedical Analysis. ,vol. 52, pp. 589- 596 ,(2010) , 10.1016/J.JPBA.2010.01.046
Miroslava Cuperlovic-Culf, Dean Ferguson, Adrian Culf, Pier Morin, Mohamed Touaibia, 1H NMR metabolomics analysis of glioblastoma subtypes: correlation between metabolomics and gene expression characteristics Journal of Biological Chemistry. ,vol. 287, pp. 20164- 20175 ,(2012) , 10.1074/JBC.M111.337196