Manipulating Cell Surface Glycoproteins by Targeting N-Glycan–Galectin Interactions

摘要: Abstract The interaction of cell surface receptors and transporters with cognate ligands depends on their concentration, distribution, organization at the cellular surface. majority are co- and/or post-translationally modified asparagine (N)-linked oligosaccharides (glycans). N -Glycan number structure combine to control concentration glycoproteins through interactions endogenous lectins such as galectins. ER/Golgi enzyme activity hexosamine pathway supply Golgi metabolites co-dependently regulate -glycan biosynthesis provide adaptive over growth differentiation. Studies in mice humans have revealed metabolic genetic dysregulation -glycosylation T-cell-mediated autoimmunity. In this chapter, we describe methods used analyze -glycan–galectin controlling distribution transporters.